Part:BBa_M36920

Zinc Promoter (ZntR regulated)

Promoter sequence with recognition site for ZntR transcriptional regulator protein. ZntR activates transcription when Zn(II) is bound. In the absence of Zinc, the ZntR protein will bind to this promoter region, causing a halt in transcription. In the presence of zinc, the ZntR protein will undergo conformation and unbind from the promoter region, allowing transcription. This part was edited from Part:BBa_K190016 by sequence changes from DNA2.0 for optimization.

Contribution from Squirrel-Beijing II 2024

ZntR and Zntp (BBa_K190016) are endogenous zinc ion sensing systems of E. coli. We utilized a dual plasmid system to test the expression levels of different promoters for ZntR. The promoter library was sourced from endogenous E. coli promoters and purchased from Ailurus.

We induced the system with 0 µM and 500 µM concentrations, respectively, and obtained their growth curves.：

By dividing the signal values after the two systems reached a steady state, we obtained the ratio of system fluorescence values, which we used to measure the strength of the different promoters in initiating the expression of zntR.

We hope this will provide a reference for other iGEM teams in the future.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]